Water is one of the most significant or vital resource for humanity and basic human right contamination of water bodies is one of the areas of major concern in the public health. The aim of our study is to determine presumptive coliform count in water samples which are collected from different sites of TMU, Moradabad, U.P., India. Most probable number test was done to detect to the coliform bacteria in drinking water sample. Samples were collected from different sites of TMU and bacterial isolate was identified by culture, morphology and biochemical characterization of bacteria. Out of 50 water sample, 16 (32%) were positive in which coliform bacteria were present whereas 34 (68%) were negative in which coliform bacteria were absent. Positive water samples were contaminated with a multiple coliform (43.75%) and single coliform (56.25%) bacteria isolate. Out of 9 single coliform bacteria Escherichia coli 4 (25%) Pseudomonas 2 (12.5%) Citrobacter 1(6.25%) and Klebsiella 2 (12.5%) we found. Out of 16 positive sample 9 (56.25%) water sample were satisfactory, 4(25%) were suspicious sample, and 3 (18.75%) were unsatisfactory sample. On the basis of the result obtained, some samples from different sites were polluted with coliform and other pathogenic bacteria. So we would like to suggest that all water sources of drinking water should be planned and conducted through the proper sanitation, regular treatment, and supervision of water sources and regular estimation of bacteria.