There were two new water sources were identified in Greater Cairo. Ain Alhaya is one of the most common springs that are subjected to continuous increase in the ground water level. Ain El-Khiala is a greenish brown spring located to the south of Ain El-Sira. During the present study, samples were collected from these regions and compared to quality of water collected from River Nile, Ismailia Canal and tap water (chlorinated treated water). The physicochemical parameters, disinfection by-products (DBPs) were measured in the collected samples in addition to microbiological examination. It was showed that quality of water was the same in River Nile and Ismailia Canal. The disinfection by chlorination is effective in water treatment and enhanced the water quality as compared to the other different sourced water although it gives various by-products. As regard to the springs, it was noticed that water quality is highly better in Ain Alhaya than Ain El-Khiala. The study concluded that there were statistical correlations among the physico-chemical, microbiological and organic measurements in different sourced water as compared to river nile and the most significant correlations were noticed among microbiological and organic measurements.