The Plants are enriched with variety of ingredients that could be used in the preparation of either synthetic or natural drugs. Thus, scientists have great interest in screening and assessing medicinal plants to promote preparation of new and advance drugs with minimal side effects as well as synergistic effect. Various pharmacological assays were performed to analyse the effectiveness of a plant toward therapeutic drugs. Lepidium as a genus is well known for its extravagant medicinal potential. This study was intended to explore the phytochemicals potential of Lepidium pinnatifidum. Dried powder form of Lepidium pinnatifidum was extracted with crude methanol and then process of fractionation was performed with order of increasing polarity to gain n - hexane fraction (LPH), chloroform fraction (LPC), ethyl acetate (LPE), butanol fraction (LPB) and aqueous fraction (LPA).The qualitative phytochemicals analysis of L. pinnatifidum showed the presence of various important pharmacologically active phytochemicals such as alkaloids, glycosides, flavonoids, tannins, saponins, coumarins, phenols, phytosterols, vitamin c, steroids, triterpenoids etc. Plant was found to be enriched with most of the assessed phytochemicals. On the basis of qualitative analysis, quantitative analysis of some important phytochemicals such as flavonoids, saponins, tannins showed that, this plant seemed a potent medicinal agent and could be used as a therapeutic drug in future.