The in vitro culture of meristeme according to several researchers is regarded as a means of cleansing the seedlings virus diseases. This work of tests of cleansing of banana by in vitro culture concerned three cultivars of banana: Bluggoe (Musa ABB), Prata (Musa AAB), Yangambi Km 5 (Musa AAA) infected by Banana Bunchy Top Virus (BBTV). Before in vitro culture, the immuno enzymologic test TAS-ELISA (Triple Antibody Sandwish Enzym-Linked Immuno Sorbing Assay) revealed that the totality of the seedlings of banana having 4 and 5 levels of Banana Bunchy Top Disease (BBTD) symptom were positive with the test. The setting in culture and three successive subcultures of the meristemes gave a median number of moves by going tube from 1.8 to 25.7 buds for Bluggoe, 1.5 to 26.8 buds for Prata and 1.7 to 28.5 buds per tube for Yangambi Km 5. These results prove that in vitro culture allows obtaining a large number of plants at a reduced time. After in vitro culture, the plants of banana resulting from this culture are cleansed with a percentage of 65.22 % for the cultivar Yangambi Km5 followed by Bluggoe with 71.40 % and Prata with 72.72 %. The in vitro culture thus remains a suitable technique for the cleansing of banana virus disease and can allow obtaining a large number of bananas without virus.