Saudi Journal of Pathology and Microbiology (SJPM)
Volume-2 | Issue-03 | 60-65
Original Research Article
Dengue Diagnostic Test for the Resource Constraint Developing World: Validity of Rapid Immunochromatographic Card Test against ELISA
Pragya Sharma, Yadu Vir Singh, Ajay Kumar, Monisha Biswas, Ashok K Sharma
Published : March 29, 2017
Abstract
Dengue infection is a serious global public health problem, and the dengue viruses are widely distributed
throughout the tropical and subtropical areas of the world. In the Indian subcontinent, the epidemiology of dengue fever
has been very complex. High prevalence rate in our region particularly in pre-monsoon and monsoon season is alarming
and necessitates early and accurate diagnosis of dengue virus infection to prevent its complications and further
morbidity. Now-a-days detection by rapid tests offers an even faster route to a presumptive dengue diagnosis. Hence, this
study aims to assess the agreement between the rapid immune-chromatographic card test (RICT) for dengue and antibody
capture ELISA, the latter being used as gold standard. A prospective study was conducted from July to November 2014
and July to November 2015 in a secondary care hospital. Probable dengue cases were diagnosed as per the WHO criteria
and RICT and ELISA were conducted on the same serum samples for the detection of NS1 antigen and IgM & IgG
antibodies. The results were analyzed. The sensitivities of RICT for NS1antigen and IgM & IgG antibodies were 100%,
89.6% and 81.3%, respectively and all showed 100% specificity. In the present study, the accuracy parameters were
comparable with ELISA. Also, RICT met the WHO ASSURED criteria, hence this study recommends introduction of
RICT in all levels of health care system for an early and accurate diagnosis and confirmation of the clinical suspicion of
dengue.