Background and Objectives: There is increasing evidence that mitochondrial dysfunction and oxidative stress may be central to both chronic periodontitis and type 2 diabetes mellitus pathogenesis. HSP 60 is a mitochondrial stress protein believed to be caused by mitochondrial dysfunction. The aim of this research was therefore to test the salivary and circulatory expression of HSP 60 in periodontitis patients. Methods: A total of 30 patients aged 35-50 were chosen and classified into two groups; (1) Healthy controls; (2) Chronic periodontitis and systemically healthy. HSP 60 in serum and saliva was estimated using a specific ELISA kit and correlated with periodontal parameters using statistical tests. Results: The serum and salivary levels of heat shock proteins were significantly higher in chronic periodontitis patients compared to healthy controls. Conclusion: Salivary levels of HSP 60 can be used as a biomarker to determine periodontitis severity. Patients with chronic periodontitis had higher salivary HSP 60 levels.