Staphylococcus aureus (S. aureus) causes a wide spectrum of clinical disorders with pathogenic factors such as various toxins. The aims of this study were analysis of the prevalence of pvl gene among MSSA, MRSA, HA-MRSA, CA-MRSA and MSSA+MRSA groups in Iran. The prevalence of Panton-valentine leukocidin in Iran was searched from searching engines such as Google, Google Scholar, PubMed, Sciverse and so on. The terms PVL, MRSA, MSSA, Iran were investigated. Data was entered in Excel and Graph Pad Prism 6 for the analysis. Both children and adults were included in the study. Twenty-one previous publications on pvl gene prevalence were found. According to the results, the most prevalence of pvl gene was in MRSA, particularly in CA-MRSA group. However, the prevalence among HAMRSA and MSSA groups was 17 and 25 percent respectively, showing a growing number of these strains encoding Panton valentine leukocidin toxin. The highest prevalence of pvl gene among previous publications was detected in CAMRSA (74%), followed by 66.26% in HA-MRSA, 60% in HA+CA MRSA, 50% in MSSA+MRSA and 40.9% in MSSA+MRSA. On the other hand, the lowest prevalence was found in MSSA (3.3%) and 6% in MSSA+MRSA. Furthermore, previous hospitalization, carrier subjects, and age of them were possible risk factors in this case. The prevalence of pvl gene is significantly higher in MRSA than MSSA (p<0.05) and likewise higher in CA-MRSA than HAMRSA strains. There is an increasing rate of presence of pvl gene in MSSA and HA-MRSA, showing transmission of related SAG prophage to methicillin susceptible and also healthcare associated strains. Previous hospitalization, carrier subjects, and age of them were possible risk factors in this case.