Background and objectives: Pit and fissure sealants have been long suggested as the method of preventing dental caries. The aim of the study was to provide an overview of the latest evidence on clinical effectiveness of pit and fissure sealants in prevention of dental caries. Materials and methods: Literature survey was carried out from 2010 to 2020 in electronic databases such as PubMed, Google Scholar and Virtual Health Library database using key words such as “Pit and fissure sealants” and “systematic reviews,”. The searches revealed 457 articles out of which 5 were selected after reading the full text articles. Results: The review of 5 articles revealed that high viscosity GIC and resin-based sealants have similar caries-preventive efficacy in permanent molar teeth after 48 months. The evidence for a potential superiority of high viscosity GIC over resin-based sealants after 60 months is still low. Sealants alone had a 2- to 3-fold higher risk of arresting or reversing lesions (moderate certainty for all comparisons) as compared to no treatment. In comparison to no therapy, the combination of sealants and 5% NaF varnish was the most effective in arresting or reversing lesions. When compared to varnish, Fissure sealants had limited superiority in minimizing occlusal caries. Conclusions: The evidence suggests that sealing pit and fissures are effective in prevention of dental caries however it is hampered by high risk of bias. Hence, future studies with high quality should be conducted assessing the effectiveness of different types of sealants.