Dengue fever is one of the most serious mosquito- borne Arboviral infections affecting tropical and subtropical countries in the world. Due to lack of immune prophylaxis and specific anti-viral therapy, outbreaks occur almost every year. There is a need for rapid diagnosis to prevent morbidity and mortality. The study was conducted to know the seroprevalance of dengue virus in a tertiary care hospital, Nellore. Blood samples received in microbiology laboratory from suspected dengue cases during June 2015 to October 2015 were serologically analyzed. NS1 Antigen, IgG and IgM antibodies were detected employing Dengue Day 1 Test kit (J.Mitra & Co. Pvt Ltd) by solid phase immunochromatographic method. Among the samples collected, 88.69% were positive for NS1 antigen, 3.41% for IgM and 2.77% for IgG. Thrombocytopenia was observed in 71.42% cases, highest being in those positive for NS1 antigen only (301 cases). Combination of antigen and antibody assays on single serum sample, ease and time taken by Immunochromatography test makes it preferable test of choice for making a reliable diagnosis.