Mustard as the medicinal plant used for the treatment of various diseases. There are 3 types of mustard: brown, oriental and yellow. Generally, the mustard seeds are mainly used in condiment or food industries in either oil or seeds form. The mustard plant is a plant species belongs to family Brassicaceae and the genera Brassica. The seed of mustard is used as spice. Derivatives of mustard constituent’s allyl iso-thiocyanate form basis for deadly agents such as anti-neoplastic drugs (e.g. bendamustine) and mustard gases. It helps for karyotyping process that centered on meiotic and mitotic chromosome and used many methods of staining comprising CMA3/DAPI fluorescence staining, Giemsa staining, silver staining, C banding, and fluorescence in situ hybridization with repetitive DNA sequences. As a source of vitamin-K plays its important role in serving with the blood clotting. It is also important and essential for the health of bone and heart. All these mustard species can also be utilized in the production of eatable oils. Both oriental and brown mustards are utilized to make the products which are spicier than the condiments which obtained from yellow mustard.