The development of a biological profile involves the estimation of sex and stature of the deceased individual usually by the comparison of the antemortem and postmortem data. Due to inadequate studies on the development of a biological profile from the craniofacial region in the Nigerian population, this study aimed to determine sex and stature from craniofacial variables in the Yoruba ethnic group of southwest Nigeria. A prospective cross-sectional study was carried out on 368 students and staff (217 males and 151 females) of the Ekiti State University, Ado-Ekiti, Nigeria within the age range of 18 – 55 years. Twelve craniofacial variables and stature were taken using standard anthropometric procedures and equipment. Data were analyzed using IBM® SPSS version 25.0, Armonk, New York, USA. Student’s t-test Correlation analysis (Karl Pearson’s), multivariate regression and discriminant function analysis were carried out. The results revealed sex differences as the males have higher mean values compared to the females except for the bizygomatic breadth. Physiognomic facial height offers a significant model for stature estimation in both male and female subjects. However, the craniofacial variables produced weak correlations with stature and are not suitable for estimating stature in the ethnic group. Multivariate regression analysis provided better results. The discriminant function analysis sufficiently discriminated sex and after cross-validation, it produced an accuracy of 86.2% for males and 89.4% for females. Sex and stature can be reliably inferred from the craniofacial region but caution should be applied as it has proven to be sex and population specific.