Background: The micronutrient folate and zinc has been associated with semen quality. Nutritional supplement with zinc and folate can help reverse some of the oxidative damage from environmental toxin and natural aging on spermatogenesis and sperm maturation. Till now there has been variable results in the research done on semen quality after folic acid and zinc sulfate supplementation. The objective of this study is to determine whether supplementation of folic acid and zinc sulfate has any beneficial effect on semen quality. Methods: This was a prospective, observational study carried out in the Department of Reproductive Endocrinology and Infertility, Bangabandhu Sheikh Mujib Medical University. Study participants are male partners of infertile couples, 70 in number, who attended the outpatient department and had abnormal semen parameters. Supplementation of folic acid and zinc sulfate was given to all male partners at a dose of 5mg tablet twice daily and 10mg tablet twice daily respectively for twelve weeks. Semen analysis was repeated after the period of three months supplementation of folic acid and zinc sulfate. Paired students t tests were performed for test of significance. Results: Oligoasthenoteratozoospermia is most common (40%) form of abnormality in semen parameter before supplementation. After supplementation 40% of report shows normozoospermia. Following supplementation of folic acid and zinc sulfate there was significant changes in sperm count (P<0.05), morphology (P<0.002), total motility (P<0.001). Regarding motility, all types of motility improved after supplementation including rapid-linear (P<0.001), slow linear (P<0.001), non-progressive (P<0.047). Number of immotile cell was reduced (P<0.001) after supplementation. Conclusion: An improvement in nutrition or supplementation with zinc and folate can have noticeable effects on semen quality and these may improve natural fertility and success rates with fertility treatment.