In order to minimize the use of expensive chemical fertilizers, this study investigate the potential of phosphate solubilization by bacterium isolated from soil which the most of the bacteria isolated from the soil have the ability to dissolve rock phosphates both in the soil and in the culture medium is well known. The microorganisms produce low molecular mass organic acids, which attack the phosphate structure and transform phosphorus from nonutilizable to the utilizable for the plants form. The objective of the present study is to study the factors affecting on dissolution of phosphate content in Abu Tartur phosphate ore by using bacterium isolated from soil. Serial dilution method was performed to inoculum solutions to achieve microorganism’s isolation which obtaining one bacterium that has ability to dissolve phosphate ore and molecular identification by 16 sRNA which suggest it called Azotobacter vinelandii. Optimum conditions of bioleaching of Abu Tartur phosphate ore are 3 days incubation period, modified PVK medium is the best medium for dissolution of Abu Tartur phosphate ore, 0.1x 1029 colony forming unit of Azotobacter vinelandii for 50 ml medium, 0.5% Abu Tartur phosphate ore concentration , 30°c, ammonium oxalate as nitrogen source, glucose as carbon source, ,no significant effect of addition factor, also there is decreasing in pH and increases in electric potential, initial pH 7, which the leaching efficiency of phosphate content in Abu Tartur phosphate ore reaches to 52.6%.