Bacterial contamination is a growing global public health threat for individuals, food industry, hospital diagnostics and society. While antibiotics can be used to treat most bacterial infections, they constantly accelerate the emergence and spread of resistance bacteria. Today, many antibiotics are ineffective, but the pace of discovery of new antibiotic has dropped substantially. Conventional methods used to detect and quantify bacteria are plate culturing, polymerase chain reaction (PCR), enzyme linked immunosorbent assay (ELISA) and chemical sensors based detection strategies. Molecular based detection method PCR is considered to be another standard method to detect bacteria. This method can be used for bacteria detection with high sensitivity and specificity, but well-trained personnel and costly instrumentations are indispensable. New colorimetric bacteria detection method based on bacterial inhibition of glucose oxidase-catalyzed reaction. The concentrations of microbes access by following the disc diffusion method that using the antibiotics and also discs to differentiate the different strains of bacteria. Bacterial cultures ability grown in petri plates by using the growth medium such as nutrient agar. Methicillin resistant Staphylococcus aureus (MRSA) is detected with excellent specificity and sensitivity by IDI-MRSA via simultaneous targeting of the staphylococcal chromosomal cassettes.