Zinc as one of the heavy metals that influence of the growth of the plant. Its deficiency in plants leads to abnormal growth of roots cells that ultimately inhibited the RNA and proteins synthesis. Sunflower has gains much important in agricultural pint of view due to its morphological characteristics. High and low concentrations of the zinc have been evaluated on the different parameters of sunflower. Water in the concentrations of 25 ml were applied carefully to the each pots and crops were harvested for evaluation of microbiological and morphological parameters such as for number of leaves of sunflower hybrids, root dry weight of sunflower, shoot dry weight of sunflower, chlorophyll pigments, carotenoids. In relation to biochemical attributes chlorophyll a and chlorophyll b were reduced while a subsequent elevation was observed in carotenoid. Maximum reduction of growth, biochemical and physiological parameters was noted at 40mM concentration of zinc. Zinc toxicity involves in inhibition of cytoplasmic enzymes and damage to cell structures due to oxidative stress. It alters the catalytic function of enzymes which damage the cellular membranes and inhibit root growth. Zinc stress cause maximum effect on hybrid FH674 as compared to other hybrid FH687.