Abstract: Essential oil of Fagara macrophylla from DR Congo was tested for antihemolytic, radical scavenging and antibacterial activities. The extraction gives an essential oil yield of 1.08 %, density of 0.7066 and refractive index of 1.4721 20°C. Aqueous and methanolic extracts showed high antisickling activity with the normalization rate (NR), the minimal concentration of normalization (MCN) and the concentration that normalized 50% of RBC (ED50) for aqueous extract of respectively 93%, 8µg/mL and 0.43µg/mL and for the methanolic extract NR, MCN and ED50 are respectively of 91%, 12µg/mL and 0.60µg/mL. The essential oil showed no antisickling activity but weak antihemolytic and radical scavenging activities. The F macrophylla essential oil showed higher bacterial activity on Staphylococcus aureus than the two antibiotics used as standards (Meropenem, Ceftriaxone).