Abstract: The aim of this study was to assess the possible risk posed by suspected contaminants in Ekerekana and Okochiri river channels on ecological and human health using a resident fish, Periophthalmodon papillo. In this study, chemical- and bio-monitoring assessments were carried out in two sampling stations, Ekerekana (EKE) and Okochiri (OKO). The chemical monitoring involved sediment and environmental water quality analysis with the goal to identify target chemicals and evaluation of environmental water quality index (EWQI) for Ekerekana and Okochiri river channels. The bio-monitoring involved the assessment of target chemicals in fish tissue for the evaluation of the edibility status of the bio-indicator fish. Fish edibility study showed that the bio-indicator fish was found to be unsafe for consumption because lead (Pb) (5,4mg/kg) and Copper (Cu) (0,99mg/kg) were beyond their oral reference dose (RfD). The FCS showed an average Fish Consumption Rate (CR) of 5.3g/day in Okochiri and 4.6g/day in Ekerekana by subsistence fishers, with an age group range of 20-29 forming the highest consumers. The HQ>1 for Pb and Cu, meaning their levels in the edible part of the study fish is of potential concern and the estimated rate of the fish consumption can be hazardous to human health. The results suggest that the EKE and OKO creeks have a negative ecological and human health impact on the affected communities. There is therefore the urgent need to check the contamination of these creeks and river system in order to avert a more serious environmental consequence.