The aim of this study was to isolate Staphylococcus aureus from samples of raw bovine milk obtained from different farms and to determine their antibiotic susceptibility patterns. A total of 144 milk samples were collected and screened for the presence of S. aureus. Antibiotic susceptibility was determined qualitatively using the Kirby-Bauer disc diffusion method. Piperacillin showed maximum resistance rate 62.90%, 61% isolates were resistant to Ceftazidime, 50% to Cephalothin, 48.38% to Gentamicin, 46.77% to Novobiocin, 43.54% to Amikacin and 40.32% to Cetoperazone. Only 9.67% of the isolates were found resistant to Erythromycin. S. aureus is normally resident in humans, therefore, the S. aureus present in cows may have resulted from transmission between the two species, emphasing the need to improve sanitary condition in the milking environment.