Reaction time (RT) is a true pointer of eye-hand coordination, response, and alertness of a person. In sports and daily life, the majority of work is done by the use of auditory and visual information. RT is a measurement of how quickly the particular tasks are done. The purpose of this study was to investigate auditory and visual simple reaction time of university male team branch athletes (basketball, handball, volleyball, football, and cricket) and non-athletes. Totally forty (40) subjects randomly participated in this study; among them (n=20) were team branch athletes and the rest (n=20) non-athletes. Audio-Visual Reaction Timer (AVRT) was used to collect auditory reaction time (ART) and visual reaction time (VRT) data. Ten values of ART and VRT of dominant and non-dominant hands were recorded. Excluding the two fastest and slowest values, and the average for the middle six values saved as two digits of milliseconds were RT data. Descriptive statistics- mean, SD, and Inferential statistics- dependent and independent t-test was applied to check the level of significance (p<0.05 and p<0.01). Paired sample t-test of university athletes and non-athletes ART was statistically significant than that of VRT (p<0.01). A significant difference was found of ART and VRT between dominant and non-dominant hands of both groups together (p<0.01). Independent t-test of ART and VRT of dominant and non-dominant hands between athletes and non-athletes was statistically significant (p<0.05). In summary, it can be said that in athletes and non-athletes groups auditory reaction time (ART) was faster than the visual reaction time (VRT) and considering both the groups together, the dominant hand was superior to the non-dominant hand in quickness. However, ART and VRT of dominant and non-dominant hands of university athletes took the upper hand over the non-athletes group.