Medicinal plants play a vital role to preserve human health. The genus Argemone consists of herbs, that are widespread in all over India and represents highly valuable plant species having therapeutic and nutraceutical importance. Genetic variation is essential for long term survival of species and it is a critical feature in conservation. Argemone mexicana (Papaveraceae) also known as Ghamoya. Traditionally the plants used the world for the treatment of several ailments including tumors, warts, skin diseases, inflammations, rheumatism, jaundice, leprosy, microbial infections, and malaria. It is an exotic weed indigenous to South America. In the present study an attempt has been made to assess the preliminary phytochemical screening and TLC profile of various leaves extract of A.mexicana.