The regulatory role of kisspeptin and its receptor, G protein coupled receptor 54 (GPR54) on reproductive processes is well documented. The main objective of this study is to determine the effects of non synonymous single nucleotide polymorphism in kiss 1 receptor gene of sheep (F 195 L). The amino acid sequence (>HM135393) was retrieved from the data bank of National Centre for Biotechnology Information (NCBI, db SNP). Analysis using panther indicated the damaging effects of substitution of Phenylalanine with Leucine at position 195 of the amino acid sequence of Kisspeptin. Effects of single nucleotide polymorphism was also predicted to be deleterious by Provean software. The study provide useful information on the potential role of Kiss 1 receptor gene in regulating reproductive traits in sheep. Findings of this research highlights the role of GPR54 as an efficient genetic marker for improving reproductive performance of farm animals.