Introduction: Intravenous catheterization or Peripheral venous catheterization is one of the most being procedures by health care providers during patient hospitalization. For the purpose of sampling, fluids, diagnostic procedure medication administration, and nutritional supplement. Phlebitis is the most common peripheral catheter related complication. .It appears to local edema and inflammation, discomfort, redness of skin or subcutaneous. Phlebitis can be chemical, mechanical and biological at the site of puncture. Objectives: Determine the associating factors of phlebitis among registered nurses on the basis of experience and nursing education. Material and Methods: This study was an analytical cross-sectional study and conducted at Peoples Medical College Hospital Nawabshah. In this study, a purposive sampling method was used, and the sample size of the study was 90 Registered nurses. Results: The gender distribution among subjects was male 50 (55.6%) and females were 40 (44. 4%). Designation among study subjects was 80(88.8%) were staff nurses and only 10(11.1%) were head nurses. A slum of subjects said antibiotics 70(77.8%) develop phlebitis, significant with experience (p=.000). The continuous I.V fluid 80(88.9%) and intermittent fluid 10(11.1%) develop phlebitis and found significant with experiences. Conclusion: The study, examined; nurses have significant awareness for the prevention of phlebitis and maintenance of peripheral I.V. Further study also found the factors associated with phlebitis.