Introduction: The Neonatal sepsis defines as blood infection that will occurs in newborn at the first four weeks from birth. Gold standard method is for diagnose of neonatal sepsis that is blood culture but that is time consuming, requires well equipped laboratory and trained laboratory personnel. The study is planned for evaluation of CRP role in the blood as most early marker in neonatal sepsis & find the correlation with blood culture. Materials And Method: The study was done in microbiology laboratory at Sir-T Hospital, Bhavnagar, Gujarat from may, 2020 to October , 2020 to understand the Correlation of C-Reactive Protein and Blood Culture in Neonatal Sepsis. After filling the form of patient’s details in pre-designed proforma the 1-2 ml of blood collected aseptically and its was inoculated into blood culture bottle containing 5 ml of Brain Heart Infusion Broth. CRP level was done by Latex Agglutination Card test. Results: Out of total 481 samples 129 were males and 352 were females. Out of the total 481 samples, 58 were blood culture positive from which 58 were positive for CRP also. Among the blood culture negative samples, 55 were CRP positive. Conclusion: There is Early diagnosis of neonatal sepsis within the aid of biomarkers like C-Reactive Protein may be serve as an important tool in the reducing mortality & morbidity among the neonates.