Spotted fever group Rickettsiae are predominantly transmitted by ticks. Rickettsiae have developed many strategies to adapt to different environmental conditions, including those within their arthropod vectors and vertebrate hosts. The tick-Rickettsiae relationship has been a point of interest for many researchers, with most studies concentrating on the role of ticks as vectors. Unfortunately, less attention has been directed towards the relationship of Rickettsiae with tick cells, tissues, and organs. This work was carried out to identify and prepare smear from salivary gland of ticks. Ticks were collected from June to September, 2018 in vegetation around residences (n= 30) of people who have been in hospital as a result of symptoms related to spotted fever. Some samples were collected from patients who diagnosed with symptoms related to spotted fever in El-Beida City. Ticks were sorted by species and life stage into pools for analyses for pathogens. Smear of salivary gland for male and female of thick were stained with gram negative bacteria and examined under microscope. Results from stains were positive for the presence of bacteria in female sample and negative for male sample.