This study we investigated the effects of insulin spray treatment on skin wounds in diabetic rats. Thirty rats were used for experiment. Rats were divided into 3 groups. group 1 serve as control group. Group 2 was diabetic without treatment. Group 3 was diabetic and treated with insulin spray daily for eight days. Diabetus was inducted by injection of alloxan at dose 100mg\kg b.w. Artificial wounds (8-mm diameter) were created in all rats on the day 3 of the experiment. Wound gaps were measured at (Zero, 2th, 4th, 6th and 8th) days post wound (pw). Animals sacrificed at 8th day (p w). Serum was collected for biochemical analyses and wound tissues were excised for histological and immunohistochemical examinations. The results showed significance increase in liver and kidneys analysis, but significance decrease in total antioxidant capacity (TAC) and superoxide dismutase (SOD) in diabetic rats when compared with healthy rats. Level of Malondialdehyde (MDA) and nitric oxide (NO) were increased in group 2 & 3 in comparison to control group. Histological findings showed contracted wounds in insulin spray group. Microscopic finding showed septic wounds in diabetic animals in contrast to insulin treated rats, which displayed re-epithelization and remodeling in wound injury at 8th day (ip). It could be concluded that insulin spray is able to accelerate the process of wound healing in diabetic rats.