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Saudi Journal of Pathology and Microbiology (SJPM)
Volume-6 | Issue-01 | 23-28
Original Research Article
Molecular Detection of Enteric Viruses in Treated Wastewater, Surface and Groundwater in Errachidia Region, Morocco
Rachid Bounagua, Hicham El Rhaffouli, Fatima El Boukhrissi, Naoufel Rahmatallah, Zakaria Yassin, Hind El Kasraoui, Abdelillah Laraqui, Coralie Portelli-Clerc, Yassine Sekhsoukh, Ouafaa Fassi-Fihri
Published : Jan. 11, 2021
DOI : 10.36348/sjpm.2021.v06i01.006
Abstract
One-year surveillance for enteric viruses in raw sewage, treated water and groundwater near of near effluents from the wastewater treatment plant (WWTPs) of Errachidia, Morocco from March 2018 to April 2019. samples were carried out in 1-liter bottles sterile propylene at the levels of raw water, treated water, and effluent water at several points and well water. Virus concentration was performed according to an end point filtration on cellulose nitrate filter of 0.2µm pore. A total of 108 was screened for Enterovirus (EV), Rotavirus (RV), Norovirus (NoV) and Hepatitis A (HAV) using a real time RT-PCR technique. The study shows a persistence of the contamination of the treated water, effluents and groundwater by EV, NoV and RV. Contrariwise, no HAV was detected in any sample performed during the study period. Detection rate for EV ranged from 100% for surface water to 50% for groundwater. For NoV detection rate ranged from 100% to 33% for surface and groundwater respectively while RV was detected in 8% to 42% in surface water and 0% to 8% in groundwater. These results suggest that enteric virus contamination of wadi Ziz negatively impact on their microbiological quality since they are used for irrigation and drinking intake, which exposes the population of the region to constant risk of acute gastroenteritis, especially in children.
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