Catharanthus rosea is an important ethno medicinal plant rich in bioactive compounds mainly alkaloids used widely in treatment of cancer disease. The aim of present study was to determine the total phenol, flavonoid, tannin and alkaloid content in leaf stem and root parts of Catharanthus rosea in benzene, methanol, aqueous, chloroform and petroleum ether extracts by spectrophotometric method. Phytochemical analysis showed presence of saponin, tannin, terpenoid, flavonoid, cardiac glycoside, alkaloid, steroid, quinones and phenolic compounds whereas protein was found absent. The total phenol, flavonoid, tannin, and alkaloid content of plant in different extracts were determined in comparison to standard gallic acid, rutin, tannic acid and quercetin respectively. The higher concentration for phenol was found in benzene leaf extract (0.65±0.02) µg/ml, flavonoid in chloroform leaf extract (0.62±0.02) µg/ml, tannin in methanol root extract (21±0.01) µg/ml and alkaloid in benzene leaf extract (0.61±0.03) µg/ml. The presence of such metabolites indicates therapeutic importance of plant.