The aim of this study was to evaluate the sensitivity of Fecal Occult Blood (FOB) strips in the diagnosis of Pulmonary Tuberculosis (PTB) isolated from sputum of tubercles patient. The study was carried out in Omdurman Teaching Hospital-Abu Anga-Tuberculosis ward. From 27th of August to 25th of September 2007. A total of 67 sputum samples collected from the suspected people. The samples examined by FOB test and Z.N techniques. Ten out of the enrolled samples, which showed positive FOB and negative Z.N techniques selected confirmed by using PCR techniques. The result showed that there were 12 (17.9%) of cases were Z.N (+ve), while 55 (82.1%) of cases were Z.N (-ve). It was also showed that there were 53(79.1%) of cases were FOB test (+ve), while 14(20.9%) of cases were FOB test (-ve). According to PCR techniques, after performed 10 samples that showed Z.N (-ve) and FOB test (+ve), it was noted that there were 4(40%) of cases were PCR (+ve), while 6(60%) of cases were PCR (-ve).