Dengue fever and Dengue Haemorrhagic fever (DF/DHF) is an acute viral disease transmitted by the bite of an infected female mosquito- Aedes aegypti. The dengue virus causes significant morbidity and mortality in many parts of the world, including India where it was first isolated in Calcutta, West Bengal during 1945. This study was conducted to know the seroprevalence of Dengue virus in a tertiary care hospital, Gujarat.Methods: Blood for serological studies are carefully collected taking due universal precautions from suspected DF/DHF cases (a) as soon as possible after hospital admission or attendance. All the patients were screened for Dengue NS1 and anti-dengue IgM antibodies by Enzyme Linked Immunosorbant Assay (ELISA). The study period was 6 months from June 2020 to December 2020.Results: From a total of 6238 serum samples 2764 tested for Dengue NS1 and IgM, 2764 (44.3%) were positive. 1606 (58.1%) were only NS1 positive and 1158 (41.9%) were IgM positive. Conclusion: Surveillance is prerequisite for monitoring the dengue situation in the area and should be carried out regularly for early detection of an impending outbreak and to initiate timely preventive and control measures. [Int J Res Med Sci 2013; 1(4.000): 448-500]