Introduction: Pregnancy is characterized by significant metabolic and hemodynamic changes that begin early in the gestational period. Major hemodynamic changes include an increase in the cardiac output during the first trimester, sodium and water retention leading to plasma volume expansion with a peak around week 30, and reductions in the systemic vascular resistance and systemic blood pressure. Material and Methods: This is prospective and observational study conducted at Department of Biochemistry in association of Department of General Medicine. In this study investigation was done to determine the contribution of different biochemical parameters of females suffering from preeclampsia and Eclampsia. Results: Serum iron level in case group 18.43 ± 8.49 μmol/L and control group 15.92 ± 8.23. Moreover, Serum Zinc level 8.4 ± 1.9 μmol/L and control group 9.3 ± 1.2 μmol/L. Furthermore, Serum Copper level 17.3 ± 5.3 μmol/L and control group 9.6 ± 1.9 μmol/L. Oxidative marker between Case and control group was statistically significantly difference. MDA in case group was 3.18 ± 0.43 nM/mL and Control 1.63 ± 0.32 nM/mL. Antioxidant marker between Case and control group was statistically significantly difference. In case group Glutathione peroxidase 9.53 ± 2.46 μmol/ml and control group 11.68 ± 3.63 μmol/ml. Conclusion: The results obtained support our initial hypothesis that common differences between all pregnant women and controls could be revealed, while preeclampsia and the other pregnancies would further differ indicating specific misbalance of the oxidative stress in preeclampsia.