Background: In stroke scenario, decreased hemoglobin levels have the potential to worsen brain ischemia; however, the association between the levels of hemoglobin with stroke mortality is not well understood. Aim: The study investigated the association of hemoglobin levels with 28 days mortality rate after ischemic stroke (IS). Methods: This was a retrospective study, where data of 74 patients admitted to ICU was collected with a diagnosis of stroke .The data were analyzed in the terms of demographic details, plasma glucose, HbA1c and hemoglobin levels. Results: Among 74 analyzed patients, 56.70% were men and 43.30% were females. In our study 32.4 % of stroke patients had anemia. Out of 45.9% non survivors, 21.6% stroke patients had hemoglobin level less than 11mg/ dl and 24.30% stroke patients had hemoglobin level more than 11mg/dl. Out of 54.1% survivors ,10.8.% and 43.20% stroke patients had hemoglobin level less than 11mg/ dl and more than 11mg/dl, respectively .The data was statistically significant. Conclusion: In conclusion, we showed that a significant proportion of stroke patients have low hemoglobin at the time of stroke onset and this is associated with increased mortality up to 28 days. Targeted interventions in this patient population may improve outcomes and require further evaluation.