The study was done in river Nile state, from September to November 2017. The research applied on 50 urine samples which collected from married ladies with young ages (18-24 years) unvaccinated against Human Papilloma Virus 16 and 18 that known as high risk viruses to detect DNA of it and probability of it to cause cervical cancer. The study aimed to detect affection of high risk HPV type 16 or 18 on young married ladies (16-18 years)an vaccinated against it Then probability to cause cervical cancer. Used molecular method to detect presence of high risk HPV DNA (16or 18) in urine sample which collected from the study group. All 50 samples were negative, that mean no detection for HPV16 or 18 DNA.