Polycystic ovary syndrome, also known as Stein-Leventhal syndrome is a common health problem that affects teenage girls and young women. PCOS is associated with a wide spectrum of complications in various parts of health, including reproductive, metabolic and psychological features. This Analytical case control study was conducted to Assessment of Lipid peroxidation, antioxidant enzyme Super oxide dismutase, Glutathione and Serum Homocysteine level in Patients with Polycystic Ovary Syndrome in Khartoum State in which include 300 participant divided into 200 case and 100 control, the sample used to measure Homocysteine , Glutathione, Lipid peroxidation and superoxide dismutase level in serum of participant of high body mass index in case group and normal body mass index for the control by using spectrophotometer machine . out of 300 sample divided into 200 case and 100 control , the mean and standard deviation of the total glutathione was found mean 801.30 and the reduced glutathione 132.2 while in control 748.6,103.2 respectively, and for Super oxide dismutase mean and standard deviation was found 225.2, ± 57.8 in case while in control found 195.5, ± 25.6 respectively and Homocysteine mean and standard deviation found 14.9, ± 2.1 for case and 12.1 , ± 2.5 for control lastly also the mean and standard deviation investigated while the lipid peroxidation was found 3.4 , ± 1.1 for case and 2.4 , ± 0.7 Respectively. the total glutathione mean and standard deviation in group of <5 years disease duration PCOS found 1559.6 ,± 282.6 and for duration of >5 years of disease the total glutathione mean and standard deviation found 1196.9 , ± 253.9 regarding superoxide dismutase mean and standard deviation in group of <5 years disease duration was found 227.1 , ± 57.4 and for duration of >5 years of disease the superoxide dismutase mean and standard deviation was found 222.4 , ± 58.8.The Homocysteine mean and standard deviation in group of <5 years disease duration was found14.9 , ± 2.2 and for duration of >5 years of PCOS disease found 14.8 , ± 1.9 The age interval have low glutathione mean and standard deviation among age of 15-30 year 60(30%) found 804.5 , ± 118.8Hemoctoseine mean and standard deviation in age 15-30 year found 15.3 , ± 2.5. Polycystic ovarian syndrome could be a dilemma for many women of reproductive ages irrespective of their reproductive disorders; should incorporate assessment of lipid profiles and possibly oxidative stress markers.