Abstract: Diabetes mellitus (DM) describes a metabolic disorder of multiple etiologies. It is characterized by chronic hyperglycemia associated with disturbances of carbohydrate, fat and protein metabolism, which result from defects in insulin secretion, insulin action. The objective of this study is to assess some of the biochemical parameters related to diabetic patients. This is a descriptive, analytic, cross-sectional, and hospital based study. The scope of this study is to assess the plasma trace elements, glycated haemoglobin among Sudanese patients with types 2 diabetes mellitus in comparison with healthy Sudanese volunteers. This study took place during the period between September 2013 to June 2016 in Khartoum state, where is the capital. Its, population is about five million inhabitants. Blood parameters were measured using spectrophotometric methods. The (mean ±SD) of glucose, HbA1, zinc and copper in healthy subjects respectively were (93.5±10.5, 4.8±0.8, 103.7±82.5,103.8±66.5). And the (mean ±SD) of glucose, HbA1, zinc and copper in patients respectively were (196.6±75.7, 7.9±2.1, 132.0±89.6, 107.0±68.6). There was significantly increased of zinc and HbA1 and also increased in means of copper in patients compared to control groups.