A clinical study has been carried out (from July to September, 2019) in order to determine the prevalence of Trypanosoma evansi infection in horses and donkeys in the Attock region of Punjab-Pakistan. A total of 100 horses (50 stallions and 50 mares) and 100 donkeys (50 jacks and 50 jennets) were examined and T. evansi was searched by the examination of wet blood film and stained thin smears (Giemsa& field staining).Eleven horses (11%, three stallions and eight mares) and 9 donkeys (9%, four jacks and five jennets) were positive respectively. However, further studies and efforts must be done by the society, government and private institutions in order to develop control measures in diseased studs/areas and to avoid dissemination of the disease.