A cross sectional study conducted during the period from February 2011 to April 2012 to determine and to evaluate the serum levels lipid profile, creatinine and HbA1c in Sudanese patients with type 2 diabetes mellitus associated with obesity. Two hundred Sudanese with type 2 diabetes mellitus associated with obesity were selected as a test group from Khartoum, Omdurman and Khartoum North hospitals in Khartoum state, Sudan. The test group was compared with a control group, which included 100 apparently healthy volunteers. Blood specimens were collected from both groups, and the serum levels of lipid profile (total cholesterol, triglycerides, high density lipoprotein cholesterol (HDLC) and low-density lipoprotein cholesterol (LDLC), in addition to fasting plasma glucose (FPG) and creatinine and glycated hemoglobin (HBA1C) were determined. Age and gender of the test group were matched with the control group. The serum levels of plasma glucose, total cholesterol, HDLC, LDLC, triglycerides, creatinine and Hba1C were measured using spectrophotometer methods. Statistical Package for Social Science (SPSS version 11.5) computer software was used for data analysis. The results of this study indicated significantly raised means of the serum levels of total cholesterol, LDLC, triglycerides and creatinine of the test group when compared with the control group, whereas the mean of the serum levels of HDLC of the test group showed a significant reduction when compared with the control group. The results of this study also indicates a significant elevation of the means of the serum levels of total cholesterol, LDLC, triglycerides and creatinine among diabetic patients with ischemic heart disease when compared with those without ischemic heart disease, whereas the mean of the serum levels of HDLC among diabetic patients with ischemic heart disease shows a significant reduction when compared with those without ischemic heart disease. The means of the serum levels of total cholesterol