According to the three-layer airport runway, based on spherical cavity-expansion theory and considering interface effect of concrete-macadam-soil by analyzing kinetic energy projectile penetrating layered target of concrete-macadam-soil, penetration models of concrete cratering, concrete pore-forming, macadam pore-forming and soil pore-forming are established. In addition, numerical solution of control equations is carried out by using Runge-Kutta method. And the Time-history curve of residual velocity is obtained. The theoretical analysis shows that because of the interface effect, the penetration resistance of projectile suffered on concrete layer and macadam layer when moving , is related not only to penetration velocity, mass, projectile shape and medium character, but also to penetration depth, which is the biggest difference compared with penetrating into single layer. Finally, by means of finite element simulation, the process of oblique penetrating into multilayered medium of airport runway is simulated by three dimensional numerical analysis. Thus, the influence of the penetration velocity and the boundary of the runway on the penetration process were studied. The results show that the presence of the multi-layer media interface of the runway accelerates the attitude of the projectile body; the smaller the penetration rate is, the more obvious the phenomenon is. At the same time, the overload response of projectile body is changed. The research results have laid a foundation for the further research on the technology of the composite medium penetration into the airport runway. They also have reference value for the research on the failure of civil engineering materials under the action of dynamic loads.