Urena lobata commonly known as Congo jute or hibiscus burr is a shrub belonging to the family Malvaceae widely distributed throughout India. The plant is utilized by the traditional healers for various ailments such as arthritis, diabetes, cough and malaria. In the present study, phytochemical constituents and antioxidant property of Urena lobata was studies in both normal extract and silver nanoparticle form. The antioxidant property of leaf of the plant was also evaluated. We have reported alkaloid, saponin, steroid, terpenoid, tannin, flavonoid and carbohydrate and significant antioxidant activity by DPPH method from this plant. For the first time, silver nanoparticle was successfully synthesized using Urena lobata leaf extract in the current investigation and characterized by UV–VIS spectrophotometer and the characteristic surface plasmon resonance peak was identified to be ⁓424 nm. X-ray diffraction (XRD) was employed to ascertain the crystalline nature and purity of the silver nanoparticles which implied the presence of 111, 002, 022, 113, and 222 lattice planes of the cubic structure of metallic silver and Fourier transform infrared spectroscopy (FTIR) was used to determine specific functional groups in silver nanoparticles and the capping agents present in the leaf extract.