Background: Diabetes mellitus is a standout amongst the most widely recognized metabolic disarranges that follow by many complications and influence many body systems and organs make them less effective and these incorporate skeletal muscles. Aim: The aim of this study is to evaluate serum creatine kinase activity in Sudanese patients with type1 and type2 diabetes mellitus in Khartoum state. Material and methods: This study was case-control study and conducted during the period from April 2018 to November 2018, 90 participants (30 type1DM, 30 type2 DM as case and 30 controls).The samples was collected by simple random sampling technique through a self-administering questionnaire, Concentration of glucose was measured for each patient with GOD method, and activity of total creatine kinase was determined also CKMB activity was measured for the tow study groups and control group as marker of cardiac functions. The analysis of data done by SPSS version 21 using T-test for comparing mean and simple correlation for correlation of continuous numerical data. Results: There was a significant increase in glucose concentration in compare to control group p-value 0.000 in both type1and type2 diabetic patient compare to control mean±SD type1 (241.9±77.5) type2 (211.3±95.1) control group (111.1±36.4). There was a significant increase in activity of total creatine kinase (CK) enzyme in type1 and type2 diabetic patients in compare to healthy individuals the p-value 0.019 and 0.010 respectively mean±SD type1 (78.0±13.2 IU/L) type2 (71.6±10.0 IU/L) and control group (58.4±23.9 IU/L). There was positive insignificant correlation between the activity of total CK and age in type1 DM patient (R-value= 0.197, P-value= 0.297), but negative insignificant correlation between the activity of total CK and age in type2 DM (R-value= -0.269, P-value= 0.151). Positive insignificant correlation between duration of diabetes and the activity of total CK level in type1 (Rvalue=0.164, P-value=0.386) and type 2 (R-value=0.158, P-value=0.403). Negative significant correlation between glucose and total CK in type1 diabetes mellitus (R-value= -0.410, P-value=0.024) and positive insignificant correlation between glucose and total CK in type2 diabetes mellitus (R-value=0.091, P-value=0.633). Conclusion: The activity of total CK among diabetic patient with type1 and type2 diabetes mellitus was higher when compare to control group, there was also significant correlation between glucose and activity of total CK in type1 diabetes mellitus.