Chlorpyrifos exposure may lead to acute toxicity at higher dose persistent health effects follow acute poisoning or from long term exposure to low dose, and developmental effects appear in fetuses and children even at very small dose. The aims and objective of this review article is to study the toxicity and biochemical effects of chlorpyrifos on different body organs of Male Sprague Dawely Rats. The danger of using chlorpyrifos persists either it is used in high amount or in low amount so it must be used when it is needed most. The administration of chlorpyrifos to rats induced testicular and cardiac toxicities significant decrease in serum testosterone, FSH and LH levels as well as the significant increase in CK-MB, LDH and AST activities. In liver and kidney tissues, dimension of glutathione and the action of superoxide dismutase and catalase chemicals altogether diminished by chlorpyrifos. Chlorpyrifos and lead acetate prompted change in serum and lipid outlines and observe oxidative pressure. Biochemical investigation demonstrated that organizations of the chlorpyrifos and deltamethrin obviously cause liver harm. Also sertoli cells and decrease in number of sperm count as the main factor for chlorpyrifos. These changes more obvious in rats: treated with higher dose of chlorpyrifos. So it is concluded that chlorpyrifos leads to such changes in living organisms included the rats as well as in humans.