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Haya: The Saudi Journal of Life Sciences (SJLS)
Volume-5 | Issue-10 | 215-219
Original Research Article
Selection of Culture Media and Laboratory Evaluation of Fungitoxicants for the Pathogen Causing Early Blight Disease of Potato
Uttam Bouri, Soumen Pal, Bholanath Mondal
Published : Oct. 29, 2020
DOI : 10.36348/sjls.2020.v05i10.004
Abstract
Early blight is the most common and devastating disease of potato (Solanum tuberosum L.) caused by Alternaria solani (Ellis & Martin) Jones and Grout. In vitro experiments were conducted during 2017-2018 to search appropriate growth medium of A. solani, as well as suitable fungi toxicant through quick screening methods. Seven different growth medium were used to culture the fungus in laboratory. Mycelial growth was very fast in Potato Dextrose Agar (PDA) followed by Richards’s Agar (RA) medium. Intermediate growth habit was recorded in Sabouraud’s Agar (SA), Czapek’s Dox Agar (CDA) and Potato Carrot Extract Agar (PCEA). The growth was very slow in Oat Meal Agar (OMA) and Malt Extract Agar (MES) at 72 hours of incubation. On bioefficacy evaluation, Infield Ayur showed very negligible effect to restrict mycelial growth and conidia formation, whereas Indofil M-45 (mancozeb) was more efficacious followed by Indofil Z-78 (zineb), Merger (tricyclazole + mancozeb) and Ishaan (chlorothalonil). Selection of most suitable culture media is essential for efficient growth of the fungus and its detail study. Quick and simple method to select effective fungicides within 48-72 hours is very much helpful for developing suitable disease management strategy.
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