Abstract: Marijuana induce alteration Hypothalamus and pituitary function and altered subsequently T3, T4 and TSH. To assess the Thyroid profile level(T3, T4, and TSH) in marijuana abuse .This case control study includes 120 Sudanese males (60 of them were marijuana abuse were considered as study group, and the other 60 healthy subjects were considered as control group ;were they do not use marijuana). All subjects were sex and age matched (age ranging between 18-60 years).Study group have addiction period between 1-37 years. This study carried in Alribatt hospital, Khartoum, from May to August 2015, serum was collected from both group and the concentration of T3, T4, and TSH were estimated using ELISA Bio TeK. There were a significant decrease in the level of the of T3, and TSH in marijuana abuse in comparsion with the control group where the (mean ± SD) was (1.25±1.77, 1.18±.69) for T3 and TSH respectively while the controls group had (mean ± SD) (1.04±.30, 1.91±1.21) for T3 and TSH respectively with p- value (0.00). But there was no significant difference in the level T4 when compared with the control group (9.99±4.16, 9.47±2.93) with p-value (0.19). This study concluded that T3 and TSH were highly significant decreased, but T4 were had insignificant change.