Abstract: Marijuana affects a variety of hormones that are regulated by hypothalamic function and it appears that the psychoactive ingredient, THC, is the major compound responsible for this action. To assess of LH, FSH, and Testosterone level in marijuana abuse. This case control study includes 60 marijuana abuses from Elribat hospital as case study. These patients are compared with age and sex matched with 60 healthy subjects as controls. All volunteers age ranging between 18-60 years. Blood samples were collected from all subjects, then serum concentration of LH, FSH, and testosterones were estimated using ELISA Bio TeK. There were a significant decrease in the level of LH, FSH and testosterone in marijuana abuse in comparison with a control group where the (mean ± SD) of LH, FSH, and Testosterone in marijuana abuse people were (4.9±1.9, 3.3±1.9, 19.3 ± 6.1) respectively while the (mean ± SD) of control group were (5.9±3.62, 6.9±3.6, 22.5±9.5) for LH, FSH, & Testosterone respectively. This study concluded that (LH, FSH and Testosterone) level were highly significant decreased in marijuana abuse.