A study was conducted in 112 diabetic foot ulcers to identify the ideal method were the infecting organisms can be identified. Two methods were followed, one by taking the specimen by swab and another by a small piece tissue using punch biopsy forceps. It is found that more positive culture was obtained by tissue culture method and surface contamination was more in the swab method.so it is better to obtain the specimen from the tissues is better than swab method.