Cervical cancer constitutes global health issue. Human Papilloma Virus Vaccine (HPV) has been associated with its prevention. Despite importance of the vaccine, studies have shown that diverse factors could impede its uptake. Therefore, this study assessed these perceived psychosocial barriers, as well as the uptake of HPV among Female Civil Servants in Delta State, Nigeria. Methods: The design was descriptive and cross-sectional. Multistage sampling technique was used to select 435 women, who consented. A structured questionnaire of 0.7 reliability coefficient was used to collect data at the Secretariat. Data were analyzed using frequency counts and the results were presented in tables and charts. Result: Mean age of participants was 326.7 years. Many, (44.4%) of them had adequate knowledge of the HPV and most (94.7%), had not vaccinated their female adolescents. Cost (54.5%) and inaccessibility (51.3%) were projected as deterring factors. Psychosocial factors isolated were taboo and On the whole, 68.0% were enthusiastic about compliance with vaccination, later. Conclusion: Human Papilloma Vaccine is the primary prevention tool that could reduce the incidence of cervical cancer gobally. Subsidizing its cost by stakeholders and inclusion of National Immunization Programme could improve uptake. In addition, creating awareness about the vaccine, could improve knowledge, therefore ensuring that perceived barriers do not deter people from utilizing services. Active counselling by nurses / midwives will reduce fears, correct misconception and enhance uptake. Ultimately, there will be reduction in the global burden of cervical cancer.