Background: Unprecedented measures have been adopted to control the rapid spread of the ongoing coronavirus disease (COVID-19) epidemic in Egypt. Pregnant women adherence to control measures is affected by their knowledge, attitudes, and practices (KAP) towards COVID-19. Aim: Aim of the study was to assess the knowledge, practice and attitudes of preventive measures against COVID-19 infection among pregnant women. Subjects& methods: Research design: Across sectional design was used. Setting: The study was carried out in two Maternal and Child Health Centers (Alnahal Medical Centre and Sheba MCH) that are affiliated to the Ministry of Health and Population in Zagazig City, Sharkia Governorate, Egypt, between the period from January 2020 to April 2020. Subjects: All eligible pregnant women who attended for antenatal care during the study period by using simple random sampling. Tools of data collection: A structured interviewing questionnaire that consists of five parts, part one, socio-demographic and obstetric data, part two sources of information on preventive measure against COVID-19 infection, part three, knowledge towards COVID-19 infection and its prevention measures, part four practice of preventive measures of COVID-19 infection and part five attitudes towards COVID-19 infection. Results: The findings of this research showed that more than half of women (57.6%) had satisfactory level of total knowledge about the preventive measure of COVID-19 infection. However, the majority of women (87.6%) had unsatisfactory level of total practice of preventive measures of COVID-19. Educational level and multiparty were factors significantly associated with poor practice of the preventive measures against coronavirus -19 infection among pregnant women. Conclusion: The study showed that most of the participants had adequate knowledge of preventive measures against COVID-19 infection but the practice of these preventive measures were poor among the participants. Recommendations: Provide continuous health educational program on practice of using preventive measures against COVID-19 infection especially among pregnant women.