Cord infection remains a significant cause of death among neonates in the developing world. Its prevention using the appropriate cleaning agent is key to ensuring the neonates stay free and safe during the period of cord care. This study was conducted to assess the awareness and use of chlorhexidine gel (CHG) in the prevention of neonatal sepsis among nurses and midwives in selected health facilities in Abeokuta. The descriptive study utilized a purposive sampling technique and a questionnaire derived from literature review to collect data from 42 respondents in three selected health facilities in Abeokuta. Findings revealed that while 31% were aware of the recommendation favoring the use of CHG for cord care, only 10% understood that the recommended strength for umbilical cord care is the aqueous solution or gel delivering 4% chlorhexidine. Prevention of umbilical cord infection should center on meticulous care of the umbilical cord with the use of chlorhexidine (CHG) which is the recommended cleaning agent. It is therefore recommended that a seminar or conference should be organized for nurses and midwives as this will increase awareness on the use of chlorhexidine gel for umbilical cord care and its benefits.