Objective: The main aim of this study was to establish psychometric properties of the subscales self-realization and health responsibility of the health-promoting lifestyle profile II tool among Kenyan university students Design: The study design was a cross-sectional analytical, that utilized quantitative methods Setting. The study was conducted in Kakamega County, located in Western Kenya. Analysis: Data were analyzed through confirmatory factor analysis, which was conducted using robust maximum likelihood estimation. The factor model was tested for validity and construct validity. Main outcome measures: subscales self-realization and health responsibility of the Health-Promoting Lifestyle Profile II Results: The items for self-realization and health responsibility had a Cronbach's alpha coefficient of 0.72 and 0.80, indicating acceptable reliability. For self-realization, the results of the Chi-square goodness of fit test were significant, χ2(27) = 251.61, p < .001, suggesting that the model did not adequately fit the data. The fit indices showed the RMSEA index was greater than .10, RMSEA = 0.20, 90% CI = [0.18, 0.22], which is indicative of a poor model fit. The CFI was less than .90, CFI = 0.52, suggesting that the model is indicative of a poor model fit. For health responsibility, the results of the Chi-square goodness of fit test were significant, χ2(20) = 272.58, p < .001, suggesting that the model did not adequately fit the data. Fit indices values showed the RMSEA index was greater than .10, RMSEA = 0.25, 90% CI = [0.22, 0.27], which is indicative of a poor model fit. The CFI was less than .90, CFI = 0.75, suggesting that the model is indicative of a poor model fit. Conclusion: In conclusion, within the limitations of this study, the results showed that confirmatory factor analysis could not well fit the items to their latent constructs.