Aim: The quality of care given by occupational therapists on sexual rehabilitation of persons with physical disabilities (PWDs) is influenced by their knowledge about, and attitude towards sexual rehabilitation. There is little information on the knowledge about, and attitude towards sexual rehabilitation of PWDs among OT practitioners in Nigeria. This study therefore assessed the knowledge and attitude of occupational therapy (OT) professionals and trainees about sexual rehabilitation of PWDs. Method: Using a purposive sampling technique, 73 OT professionals and trainees responded to this cross-sectional survey. Data obtained were analyzed using descriptive and inferential statistics. Significance level was set at 0.05. Results: The mean knowledge score for the OT trainees was 22.87±3.90 and 23.44±4.84 for OT professionals. Similarly, the mean score for attitude was 57.00±4.65 for OT trainees and 56.24±6.94 for OT professionals. Over 80% of the respondents had moderate knowledge about sexual rehabilitation while all the respondents had positive attitude towards sexual rehabilitation. Also, the attitude of OT professionals towards sexual rehabilitation of PWDs was significantly lower than that of the OT trainees (t=0.56; p-0.009) (Table-3). In addition, there was significant correlation between the knowledge and attitude of the respondents towards sexual rehabilitation of PWDs (ρ=0.26; p=0.025) (Table-4). Conclusion: In Nigeria, OT professionals and trainees demonstrated satisfactory knowledge and attitude about sexual rehabilitation of PWDs. Significant relationships exist between knowledge and attitude towards sexual rehabilitation of PWDs.