Nurses’ awareness of professional rights and values and how those values affect their behavior is an integral part of nursing care. This study aimed to assess the perception of married nurses regarding women’s rights and obligations and also to assess the family support towards nursing services and professional development in selected tertiary hospital of West Bengal. This descriptive survey was conducted with 103 married nurses employed in North Bengal Medical College and Hospital who were selected by non probability, total enumerative sampling technique. Data were collected using investigator prepared rating scale and the responses were self reported by the participants. The data were analyzed by descriptive statistics, associations between socio demographic variables and mean scores were analyzed by inferential statistics like chi square test. The results revealed that 46% of the nurses had average perception about their professional rights but 73% of them had good perception about their personal rights, 62% of the nurses had good perception about their professional obligations related to patient care and only 16% of the nurses had good family support towards nursing services and professional development. It was also evident that age, duration of service and type of family (χ 2 values = 4.133, 7.433, 7.336 respectively) at 0.05 level of significance were associated with perception of women’s rights and obligations. Family support was associated with professional qualification of nurses, monthly family income, type of family and working hours (χ 2 values = 4.307, 6.26, 7.01, 6.448 respectively) at 0.05 level of significance. Further studies using standardized tools and other reliable and culture specific instruments are recommended.